Research Paper Fusarium Wilt on Abaca Essay Example

Research Paper Fusarium Wilt on Abaca Paper Induction of Resistance in Abaca Against Wilt Disease Complex caused by Fusarium oxysporum f. sp. cubense (E. F. Smith) and Ralstonia solanacearum (Smith) Yabuuchi et. al. Using Chemical and Biological Elicitors Abstract Resistance in plants has been reported to be inducible using biological and or chemical elicitors. Fusarium wilt and bacterial wilt diseases are two major diseases hampering abaca production in the Philippines. This study was conducted to evaluate the efficacy of biological and chemical eliciors of resistance to control wilt disease complex in abaca caused by Fusarium oxysporum f. sp. ubense (FOC) and Ralstonia solanacearum. Suspected biological elicitors (Fomes and Xanthomonas oryzae pv. oryzae or Xoo) and known chemical elicitors (Asprin or Acetyl Salicylic acid and Boost) were sprayed to the abaca tissue cultured seedlings at 15 days interval together with water and Nordox (fungicide with bactericidal activity) as checks. The sprayed seedlings were challenged by s imultaneous inoculation with FOC and Ralstonia solanacearum. The parameters used to compare the treatments were: percent infected plants, disease severity rating, percent area of vascular discoloration and number of remaining live plants. Results show that Boost and Fomes resulted to an effective control of the wilt disease complex which were comparable or even better compared to Nordox, the fungicide check. Xoo and Acetyl Salicylic Acid on the other hand produced higher disease compared to the control. This study has demonstrated the potential of Fomes and Boost as biological and chemical elicitors of resistance, respectively in abaca which protected it from wilt disease complex. Keywords: Abaca, SAR, chemical elicitors, biological elicitors, Fusarium wilt, Bacterial wilt - Student Researcher, VSU 2 Associate Professor, DPM, VSU and Research Adviser INTRODUCTION Abaca (Musa textilis Nee) is indigenous to the Philippines, and is one of the major dollar earners of our country. Abaca plant has many slender stalks, upright and pointed leaves. It is harvested for its strong but flexible fiber usually called as Manila hemp used for making twines, ropes, cordage, pulp and specialty papers, fiber crafts, textile and fabrics. It is also one of the few cash crops that can grow with relatively little input compared to the other crops (FIDA, 2007). We will write a custom essay sample on Research Paper Fusarium Wilt on Abaca specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Research Paper Fusarium Wilt on Abaca specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Research Paper Fusarium Wilt on Abaca specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Abaca has a high market potential but its production has been limited due to the occurrence of diseases, such as bunchy top, bacterial wilt and fusarium wilt which have taken their toll on many abaca farms. This devastation caused by viruses, bacterial wilt and fusarium wilt in abaca aggravates the farmers’ production problems. Fusarium wilt disease is caused Fusarium oxysporum f. sp. cubense (E. F. Smith) Snyd. And Hans. which is also referred to as Agent Green. This fungus does so by colonizing the water-conducting vessels (xylem) of the abaca plant resulting for a blockage and breakdown of xylem. Yellowing of the leaves, followed by drying and wilting and eventually plant death are the external symptoms of the disease. Most affected plants have discolored vascular bundles and eventually die before maturity thus, forcing the farmers to harvest the abaca plant prematurely. Another equally important disease is the bacterial wilt disease. Bacterial wilt is caused by the bacteria Ralstonia solanacearum (Smith) Yabuuchi et al. , 1995). The early symptoms of this disease include rusty brown streaks in the leaves which later turn blighted and water soaked and finally wilting of the whole plant. The internal symptoms are the vascular discoloration, water soaking and finally the rotting of pseudo- stem. Fusarium wilt and bacterial wilt may occur singly or together as a complex in the field and had contributed much to the devastation of abaca in major growing areas in the Philippines. Host plant resistance is the most effective means of controlling wilt diseases. But the development of a resistant plant however takes time, effort and many resources. Resistance in plants however can be induced using chemical and biological elicitors. Several studies have shown that certain chemicals can induce the resistance of plants and some of which are boost and salicylic acid. Boost is a novel synthetic chemical, benzo(1,2,3)thiadiazole-7-8-carbothioic acid S-methyl ester (BTH) was intially shown to induce acquired resistance in wheat (Gorlach, et al, 1996) and which is produced by Novartis and distributed in the Philippines by Syngenta. Aspirin or acetyl salicylic acid was also found to induce resistance in some plants (Gleason, 1999; Owen, 1996). Salicylic acid (SA) has been reported to be elevated on plants that exhibit SAR for example in cucumber (Metraux, et al, 1990), tobacco (Malamy, et al, 1990), and Arabidopsis (Uknes, et al, 1993). There are evidences that suggest that SA plays an important role in the transduction pathway leading to SAR. Recent studies however indicate that SA is not likely to be the systematically transmitted signal (Vernooij, et al, 1994). ] There are also biotic elicitors that have been reported to elicit resistance in plants which includes chitin and chitosan. Chitin is a safe material and was reported to induce resistance against soil-borne diseases ((Kuchitsu, et al, 1993). Moreover, chitin was reported to be used as soil fertilizer. Chitosan which can be extracted from chitin has anti-fungal activity against many plant pathogens (Herano, et al, 1990; El-mougy, et al, 2002). Moreover, chitosan is also reported to induce resistance against soil-borne fungi. Chitosan was also found to have anti-bacterial property and is capable of inducing resistance to the bacterial blight disease of rice cause Xanthomonas oryzae pv. oryzae (Modina, 2007). Boost is a novel synthetic chemical, benzo(1,2,3)thiadiazole-7-8-carbothioic acid S-methyl ester (BTH) was intially shown to induce acquired resistance in wheat (Gorlach, et al, 1996) and which is produced by Novartis and distributed in the Philippines by Syngenta. Aspirin or acetyl salicylic acid was also found to induce resistance in some plants (Gleason, 1999; Owen, 1996). Salicylic acid (SA) has been reported to be elevated on plants that exhibit SAR for example in cucumber (Metraux, et al, 1990), tobacco (Malamy, et al, 1990), and Arabidopsis (Uknes, et al, 1993). There are evidences that suggest that SA lays an important role in the transduction pathway leading to SAR. Recent studies however indicate that SA is not likely to be the systematically transmitted signal (Vernooij, et al, 1994). ] This study therefore aimed to compare and contrast the efficacy of suspected elicitors of resistance in controlling the wilt disease complex in abaca. The efficacy of potential biological a nd chemical elicitors of systemic acquired resistance to control wilt disease complex of abaca caused by Fusarium oxysporum f. sp. cubense and Ralstonia solanacearum was also evaluated. METHODOLOGY Preparation and Sterilization of Culture Media Potato dextrose agar (PDA) for the culture of FOC was prepared by dissolving 39 grams dehydrated PDA to 1 liter dH2O in a double boiler and cooked until the agar dissolves in H2O. It was dispensed to 250 ml Erlenmeyer Flasks and test tubes. Nutrient agar for the culture of Ralstonia solanacearum was prepared by dissolving 3g beef extract, 5 grams peptone and 17g agar to 1 liter dH2O in a double boiler and cooked until agar dissolves in H2O. It was dispensed to 250 Erlenmeyer Flasks and test tubes. The media were sterilized in a pressure cooker at 15 lbs/in2 pressure or 121 °C temperature for 15 minutes. Sterile media were allowed to cool. PDA and NA in tubes were slanted and stored in the refrigerator until use. Collection, Isolation and Culture of FOC Isolation plates were prepared by melting the stored PDA in flasks, pouring into sterile Petri plates and allowed to solidify. Fusarium wilt– infected abaca was collected from the National Abaca Research Center and it was brought to the Plant Disease Diagnostic Laboratory for isolation of the pathogen. Infected tissues from the advancing portion of the disease were disinfected with 1% sodium hypochlorite and were planted onto solidified PDA in plates (4 sections per plate). After 2 or more days, mycelia that have radiated from the planted abaca tissue were cut aseptically using flamed sterilized flattened needle and were transferred to PDA slants where they were stored and maintained until use. Collection, Isolation and Culture of Ralstonia solanacearum Nutrient agar in flasks were melted and aseptically poured to Petri plates. The medium were allowed to solidify. The bacterial wilt pathogen, R. solanacaerum was isolated from a banana fruit infected with â€Å"Bugtok† disease. Infected tissues from the advancing portion of the disease were cut, disinfected with 1% hypochlorite and reused with sterile H20. The sections were then placed in sterile water in a test tube and allowed to stand for 10 minutes to allow the bacteria to ooze out of the tissue. A loopful of the resulting bacterial suspension was streaked onto the solidified NA on plates and incubated. After 2 days individual colonies were picked with sterile wire loop and transferred to NA slants to purify. The cultures were incubated for 2 days and stored in the refrigerator for future use. Procurement and Preparation of Test Plants Four weeks old tissue cultured abaca plantlets were procured from National Abaca Research Center (NARC). They were maintained in the vicinity of the Department of Pest Management and were given necessary care and maintenance such as watering, weeding, and fertilization. The plants were numbered and tagged according to the desired treatments. In numbering and tagging the plants, we gave them equal chances in belonging to a group. We did this through the drawn by lot method. Thus, we have used the technique randomization in giving names to the plants. Preparation and Application of Treatments The plants were sprayed with the different treatments (Fig. 1) at 15 days interval starting when the plants were 6 weeks old. Treatments| Illustration| T0 (Water)| | T1 (Fomes at 100ppm)| | T2 (Attenuated Xanthomonas oryzae pv. oryzae)| | T3 (Aspirin or Acetyl Salicylic Acid at 100ppm)| | T4 (Boost at 100ppm)| | T5 (Nordox at 100ppm)| | Fig. 1. The different treatments used Fomes was prepared by oven drying (50? C for 3 days) and by pulverizing using mortar and pestle. After pulverizing, 0. 75g was obtained and was mixed with 750ml H20 to make a 100 ppm suspension. Xanthomonas oryzae pv. oryzae (Xoo) was prepared by transferring an isolate of Xoo (courtesy of PDDL at VSU) in NA slants 2 days before its scheduled time of application. On the application time, 10ml sterile H20 was poured on the test tube slants and the bacteria were scraped using a wire loop without causing damage to the medium. Ten slants were used and the solution was diluted into a beaker to make 100 ml suspension. An approximately 650 ml H20 was added to have a total of 750 ml suspension. The suspension was heated for 5 minutes to attenuate the bacterium. Aspirin or Acetyl Salicylic Acid was prepared by making a stock solution. A tablet of aspirin was diluted to 1L H20 to make a 300 ppm suspension. Then, a volume of 37. 5ml was measured out from the stock and was added to 712. ml H20 to make a 100 ppm suspension. Boost was obtained from Syngenta Philippines. Two ml Boost was diluted and mixed to 1998 ml H20 to make a 2000 ppm suspension. A volume of 37. 5 ml was obtained out from the stock and was added to 712. 5 ml H20 to make a 100 ppm suspension. Nordox, copper-based fungicide with bactericidal activity was used as the positive check at the manufacturer’s recommended rate. It was prepared by weighing 1. 9g of Nordox and by mixing it thoroughly to 750 ml H2O. After preparing, the treatments were sprayed to the plants using a sprayit gun powered by an electric compressor starting from Water down to Nordox. Each plant equally received 50ml of the said treatments (Fig. 2). The experiment was laid in a Completely Randomized Design. The six treatments were replicated 3 times and 5 plantlets constituted one replicate making a total of 90 plantlets needed for the experiment. Two consecutive spray applications of the treatments at 15 days interval were done before pathogen inoculation and sprayings were done until the last disease scoring. Fig. 2 Application of treatments to the plants Preparation of FOC Inoculum The pure cultures were first examined under the microscope to confirm the presence of real FOC pathogen (Fig. ) before they were mass cultured for inoculation. FOC that was used for inoculation to test plants was cultured and mass produced in corn meal-sand medium (CMS) which was prepared by mixing 1 part ground corn every 20 parts of sand. The mixture was placed in glass jars about 2/3 full and moistened and covered with aluminum foil then bound with rubber band at rim (Fig 4). They were sterilized at 15 lbs/in2 pressure and 121 °C temperature for 1 hour. Pure culture of FOC from the PDA slant were aseptically seeded into the prepared CMS medium and incubated at room temperature until completely colonized by the fungus. Fig 3. Photomicrograph of FOC macroconidia and microconidia Fig 4. Corn meal-sand medium with FOC Inoculation of FOC CMS cultures of FOC were first mixed thoroughly on a tray to assure even distribution of the fungal spores to the CMS medium before inoculation. FOC was inoculated into the soil of each potted plant by incorporating 1 tbsp of the inoculum near the base of the abaca plantlet and covering the inoculum with soil. Inoculation was done 2 days after the second spraying of the different treatments. Preparation of Ralstonia solanacearum, the bacterial wilt pathogen Bacterial wilt pathogen was transferred to fresh NA slants. Two days after the isolation, each NA culture was mixed with 10ml dH2O and the pathogen was scraped using a wire loop without causing damage to the medium. Overall, 120ml of the bacterial suspension was used. One ml was injected to the stem of each plant. Bacterial Inoculation was done 12 days after FOC inoculation. Data Gathered The following data were gathered: A. Initial and Final Plant Height – This was gathered per plant basis before the application of treatments and inoculation of the pathogen and during the termination of the study. B. Percent infection – (No. of plants showing foliage yellowing or wilting / Total No. of plants) x 100 starting 1 week after inoculation and weekly until most of the plants have died in any of the treatments. C. Visual Disease Severity Rating – This was gathered using a devised scale below: We gathered this data at 1 week interval until the termination of the study. Rating| Description| 0| No wilting| 1| Slight yellowing of lower leaves| 2| Yellowing of most leaves but no dried leaves yet| 3| Extensive yellowing and moderate wilting| 4| Extensive wilting but plant still alive| | Plant totally dead| D. Percent Area of Vascular Discoloration – This was taken during the termination of the study wherein all the plants were uprooted and the pseudostems were sliced longitudinally (Fig 5). Each plant was rated using the following scale (Borines et al. 2007) Rating | Description| 0-1| No discoloration| 1-2| Isolated points of vascular discoloration| 2. 1-3| Discoloratio n up to 1/3 of vascular tissue| 3. 1-4| Discoloration between 1/3 – 2/3 of vascular tissue| 4. 1-5| Discoloration greater than 2/3 of vascular tissue| 5. -6| Total discoloration of vascular tissue| Fig. 5 Slicing of pseudostem longitudinally E. No. of surviving plants per replicate per treatment. (Gathered at the termination of the experiment. ) Data gathered were analyzed using simple CRD with three replications. Treatments were compared using Duncan’s Multiple Range Tests (DMRT). 9) Disposal of Inoculated Plants After the experiment, the inoculated plants were exposed to direct sunlight and burned when dried. RESULTS and DISCUSSION Table 1 shows the percent infected plants at 14, 21, and 28 days after inoculation. Based on the results, Boost produced significantly lower percentage infected plants compared to the control especially at 14 days after inoculation. At 21 and 28 days after inoculation, Boost still produced lower disease incidence although not statistically different from the control. Fomes, Boost and Nordox also showed lesser disease incidence values although not significantly different from the control. Table 1. Percent infected plants at 14, 21 and 28 days after inoculation Treatment| Percent Infected Plants| Days After Inoculation| 14 days| 21 days| 28 days| T0 (Control)| 73. 3 ab| 93. 33| 100| T1 (Fomes)| 66. 67 ab| 73. 33| 73. 33| T2 (Xoo)| 100. 00 a| 100. 00| 100| T3 (Aspirin)| 73. 33 ab| 100. 00| 100| T4 (Boost)| 26. 67 c| 60. 00| 80| T5 (Nordox)| 53. 33 bc| 66. 67| 83. 33| CV(%)| 26. 91| 26. 27| 21. 60| Means followed by a common letter are not significantly different at 5% DMRT. Another parameter used to measure the effect of the treatments was the mean disease severity rat ing per plant (Table 2). Based on this parameter, Boost, Nordox and Fomes have lower means of disease severity compared to the Control especially at 21 days after inoculation. Xoo and Acetyl Salicylic Acid on the other hand have significantly higher means compared to Water. Thus the two treatments have even triggered the increase in wilt disease severity ratings of the plants. Table 2. Mean wilt severity rating per plant as affected by the different treatments Treatment| Mean Disease Severity Rating| Days After Inoculation| 14 days| 21 days| 28 days| T0 (Control)| 0. 93 c| 2. 47 ab| 3. 13 ab| T1 (Fomes)| 0. 80 c| 1. 53 bc| 2. 27 b| T2 (Xoo)| 2. 87 a| 3. 45 a| 3. 92 ab| T3 (Aspirin. )| 2. 07 b| 3. 20 a| 4. 33 a| T4 (Boost)| 0. 40 c | 1. 27 c| 2. 3 b| T5 (Nordox)| 0. 73 c| 1. 07 c| 2. 47 b| CV (%)| 27. 6| 25. 7| 27. 6| Means followed by a common letter are not significantly different at 5% DMRT. The percent area of vascular discoloration per plant is shown in Table 3. This was gathered upon termination of the study. All plants were uprooted and the pseudostems were sliced longitudinally. This actually shows the internal manifestations of disease and effects of the treatments to the plants. Based on statistical analysis, Boost still consistently showed lesser percent area of vascular discoloration compared to Water (Fig. ) which further confirm its effectiveness in controlling the wilt disease complex. Fomes and Nordox also showed much lower percent area of vascular discoloration compared to control, Xoo and Aspirin. Table No. 3 Percent Area of Vascular Discoloration Treatment| Percent area of vascular discoloration| No. of Live Plants Remaining| T0 (Control)| 55. 27 ab| 3. 00 ab| T1 (Fomes)| 38. 47 b | 3. 33 ab| T2 (Xoo)| 79. 27 a| 2. 00 bc| T3 (Aspirin)| 80. 27 a| 1. 33 c| T4 (Boost)| 37. 67 b| 4. 00 a| T5 (Nordox)| 38. 47 ab| 3. 33 ab| CV (%)| 31. 37| 52. 18| Means followed by a common letter are not significantly different at 5% DMRT Fig. 6 Internal Vascular Discoloration of Plants (left-right) Control, Fomes, Xoo, Salicylic Acid, Boost, Nordox The number of live plants remaining was also gathered during the termination of the study and shown in Table 3. Boost, had the highest average number of remaining live plants followed by Fomes and Nordox. Aspirin had the lowest no. of live plants or highest mortality which was even higher than that of water (Fig. 8d, p. 24). The height of abaca plants is shown in Fig. 7. Based on the graph, the plants sprayed with Boost and Nordox have higher plant height compared to Conrol, Xoo, Fomes, and Aspirin. Among the parameters used to measure the effects of the different treatments, the mean disease severity rating on a per plant basis and mean percent area of vascular discoloration were able to differentiate more the effect of the different treatments and were considered more reliable compared to the rest of the parameters used. Fig. 7 Height of Abaca Plants at the start of the experiment, at 7, 14, 21 days and the final height before the termination of the study b c d ef Fig. 8. Group of abaca plants treated with a) water, b) Fomes, c) Xoo, d) Aspirin, e) Boost and f) Nordox. Boost is a commercially available elicitor of resistance in plants and it was found very effective in controlling the wilt disease complex in abaca. Boost, however is a little expensive, one spray cost P0. 65. Nordox is a commercial fungicide with bactericidal activity and it has als o reduced the wilt disease incidence and severity. This is a little cheaper (P0. 45) compared to Boost. A cost-effective control for the wilt disease complex was Fomes. It was able also to reduce disease incidence and severity. Although not as effective as Boost but, no cost has been incurred, except for the labor in gathering, drying and pulverizing. The use of Fomes as a biological elicitor of resistance in abaca to control wilt disease complex has been explored in this research. Most probably, being a fungus, the chitin in Fomes could be the elicitor of resistance. Kutchitsu et al (1993) reported that chitin induced resistance of plants. The active component of Fomes, that could elicit resistance in plants could be isolated in future research endeavor. CONCLUSION AND RECOMMENDATIONS Boost (chemical elicitor) and Fomes (potential biological elicitor and Nordox (fungicide with bactericidal activity), were able to effectively control the fusarium wilt and bacterial wilt disease complex of abaca. Xoo and Aspirin were not able to control the disease but instead has aggravated the wilting of the plants. A follow-up study maybe conducted to re-evaluate the same elicitors for their effect on either fusarium wilt or bacterial wilt alone. Likewise, a study maybe conducted to further evaluate the potential of Fomes as biological elicitors of resistance to other diseases of abaca or to other plant diseases. Furthermore, several other indigenously occurring biological elicitors of resistance may be evaluated against the wilt diseases of abaca. ACKNOWLEDGEMENT First and foremost, we would like to extend our gratitude to all the people behind the success of this study. We would like to thank our adviser, Dr. Lucia Borines for sharing to us her knowledge, skills effort and even her time though with a hectic schedule. To Ms. Christine Advincula, Ms. Victoria Palermo and Ms. Robleyn Piamonte, thank you so much for patiently guiding and helping us everytime we need assistance especially when our adviser is on travel. Thank you also to Mr. George Valenzona, for the analysis of our data, and Mr. Marlon Tambis and Mr. Victor Calunangan for the computer assistance. In addition, our heartfelt thanks is extended to our loving parents who supported us in our financial needs and for enlightening our faith that we could be able to finish this research on time. No words indeed can express how much we owe you this success. REFERENCES 1) BASTASA, G. N. and A. A. BALIAD. 2005. Biological control of fusarium wilt of abaca (Fusarium oxysporum) with Trichoderma and Yeast. http://cropscience Philippines. blogspot. om/2006/032005-vol30-no-2-v30n02p01-02. html 2) BORINES, L. M. 2008. Efficacy of chemical activators of systemic acquired resistance for the control of bacterial blight and diseases of rice. Paper presented during the 2008 RD Regional Conference held at Baybayon ni Agalon, Albuera Leyte, June 17-18, 2008. 3) BORINES, L. M. SALAMAT, E. E. CARDINES, R. B. 2007. Reaction of abaca (Musa textiles Nee) access ions and varieties to Fusarium wilt caused by Fusarium oxysporum f. sp. cubense. (E. F. Smith) Snyd. And Hanns. Annals of Tropical Research. Volume 29 (No. 1). 4) EL-MOUGY, N. S. , R. ABB-L-KAREEM and M. A. ABB-ALLA. 2002. Postharvest Diseases Control: Preventive Effect of Chitosan and Bioagents Against Green and Gray Moulds of Apple Fruits. Egypt. J. Phytopathol. , 30: 99-113. 5) FIDA. 2007. http://fida. da. gov. ph/Abaca6. html/ 6) GLEASON, M. 1999. Sick Plants take aspirin. http://www. extension. iastate. edu/newsrel/1999/jan99/jan9901. htm 7) GORLACH, J. , S. VOLRATH, G. KNAUF-BEITER, G. HENGY, and U, BECKHOVE, K. H. KOGEN M. OOSTENDORP, T. STAUB, E. WARD and H. KESSMANN. 1996. Benzothiadiazole, a novel class of inducers of systematic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8:629-643. 8) HERANO, S. , C. ITAKURA, H. SEINO, Y. AKIYAMA,I. NOTATA, N. KANBARA and N. KAWAKAMI. (1990). Chitosan as an Ingredient for Domestic Animal Feeds. J. Agric. Food Chem. , 38:1214-1217. 9) KUTCHITSU, K. , M. KIKUYAMA and N. SHIBUYA. 1993. N-acetylchito-oligosaccharides, Biotic Elicitor for Phytoalexin Production, Induced Transient Membrane Depolarization in Suspension-Cultured Rice Cells. Protoplasma. 174:79-81. 10) MALAMY, J. , CARR, J. P. , KLESSIG, D. F. , and RASKIN, I. (1990). Salicylic Acid: A Likely Endogenous Signal in the Resistance Response of Tobacco to Viral Infection. Science 250,1002-1004. 11) METRAUX, J. -P. , SIGNER, H. , RYALS, J. , WARD, E. , WYSS-BENZ, M. , GAUDIN. J. , RASCHDORF, K. , SCHMID, E. , BLUM, W. ,and INVERARDI, B. (1990). Increase in Salicylic Acid at the Onset of Systemic Acquired Resistance in Cucumber. Science 250, 1004-1006. 12) MODINA, IVY M. 2007. Antimicrobial Property of Chitosan and Induction of Systemic Acquired Resistance for the control of Rice Bacterial Blight caused by Xanthomonas oryzae pv. oryzae (Swings et al. ). B. S. Thesis, Visayas State University, Visca, Baybay City, Leyte. 38pp. 13) OWEN, M. 1996. Plants feeling under Weather? Give them Aspirin Water. http://www. plantea. com/plant-aspirin. htm 14) UKNES, S. , WINTER, A. , DELANEY, T. , VERNOOIJ, B. , MORSE, A. , FRIEDRICH, L. , NYE, G. , POTTER, S. , WARD, E. , and RYALS, J. (1993). Biological Induction of Systemic Acquired Resistance in Arabidopsis. Mol. Plant-Microbe Interact. 6,692-698. 15) VERNOOIJ. B. , FRIEDRICH, L. , MORSE, A. , REIST, R. , KOLDITZ-JAWHAR, R. , WARD, E. , UKNES, S. , KESSMANN, H. , and RYALS, J. (1994). Salicylic Acid is not the Translocated Signal Responsible for Inducing Systemic Acquired Resistance but is Required in Signal Transduction. Plant Cell 6,959-965. .

Amelia Earhart Family Tree

Amelia Earhart Family Tree Back to Generations 1-3 Fourth Generation (Great-Grandparents of Amelia Earhart): 8. David EARHART was born in Dec 1789 in York, Pennsylvania. He died on 3 Jun 1848 in Leechburg, Armstrong County, PA and is buried in Oakdale Cemetery, Davenport, Iowa. David EARHART and Catherine ALTMANN were married on 3 Sep 1814 in Blacklick Township, Indiana County, Pennsylvania. 9. Catherine ALTMANN was born on 12 Jun 1789 in Pennsylvania. She died on 15 Mar 1870 in Davenport, Iowa. David EARHART and Catherine ALTMANN had the following children: i. Phillip EARHART was born on 28 Mar 1815 in Indiana County, Pennsylvania. He died on 24 Dec 1904.ii. John EARHART was born on 12 Sep 1816 in Indiana County.4 iii. Rev. David EARHARTiv. Henry EARHART was born on 3 May 1819 in Pennsylvania. He died on 9 Sep 1906.v. Lucy EARHART was born on 7 Feb 1821 in Indiana County. She died on 1 Jun 1907 in Atchison, Atchison County, Kansas.vi. Daniel EARHART was born on 14 Oct 1822 in Indiana County. He died on 13 Jul 1916 in Clinton, Ohio.vii. William EARHART was born on 3 Apr 1824 in Indiana County. He died on 10 Apr 1866.viii. Samuel EARHART was born on 7 Oct 1825 in Indiana County. He died on 27 Apr 1851 in Davenport, Iowa.ix. Joseph EARHART was born on 10 Mar 1827 in Indiana County, Pennsylvania.x. Mary EARHART was born on 6 Mar 1830 in Indiana County. She died on 16 Mar 1899.xi. Robert Nixon EARHART was born on 9 Apr 1833 in Indiana County. He died on 29 Jul 1907 in Davenport, Iowa. 10. John PATTON was born btw 22 July 1793 and 21 July 1794 in Indiana County, Pennsylvania.  He died on 21 Jul 1836 in Somerset, Somerset County, Pennsylvania  and is buried in Ankeny Square Burial Ground, Somerset.   11. Harriet WELLS was born between  9 Apr 1800 and 8 Apr 1801 in Somerset, Somerset County, Pennsylvania.  She died on 9 Apr 1890 in Somerset  and is buried in Ankeny Square Burial Ground, Somerset. John PATTON and Harriet WELLS were married and had the following children: 5 i. Mary Wells PATTON 12. Isaac OTIS was born on 26 Sep 1798 in Saratoga Springs, NY. He died of an accidental injury on 12 Mar 1853 near Prairieville, Barry County, Michigan. 13. Caroline Abigail CURTISS was born on 20 Aug 1808.  She died on 12 Mar 1883 in Kalamazoo, MI. Isaac OTIS and Caroline Abigail CURTISS were married in 1826 in Homer, Cortland County, New York and  had the following children: 6 i. Judge Alfred Gideon OTISii. Charles E. OTISiii. George L. OTISiv. Ephraim A. OTISv. Isaac Newton OTISvi. Stephen OTISvii. Mary OTISviii. Louise OTISix. Lilly OTISx. Curtiss OTISxi. Arthur OTIS 14. Gephard HARRES was born on 11 Jun 1801 in Brunswick, Lower Saxony, Germany. He died on 31 May 1863 in Atchison, Atchison County, Kansas  and is buried in 1863 in Laurel Hill Cemetery, Philadelphia, Pennsylvania.   15. Maria GRACE  was born on 2 Aug 1797 in Germantown, Pennsylvania.  She died on 17 Sep 1896 in Atchison, Kansas. Gephard HARRES and Maria GRACE were married on 17 Oct 1824 in Philadelphia and  had the following children: 7 i. Amelia Josephine HARRESii. Elizabeth HARRESiii. George HARRESiv. Eliza HARRESv. John Henry HARRESvi. Charles Gebhard HARRESvii. Mary Ann HARRES was born on 29 May 1830 in Philadelphia, PA.  She died on 30 Apr 1909 in Atchison, Kansas.

Jews during emigration to three jewish songs Essay

Jews during emigration to three jewish songs - Essay Example In the letter to the editor there are four themes which are expressed by the writer which can also be found in the four Yiddish songs: the value of studying Torah; the value of returning to one's roots; the loneliness when one loses his love whether it be spiritual or a loved one and the fear of getting old and not being able to study . It must be noted that each of the songs deal with only part of the themes. The purpose of this paper is to present the themes of the songs in terms of the letter of the man who wanted to return to Lithuania to study. As children studying at their school does not compare to a 30 year old man, what they learn from studying is the same. Losing one's wife is difficult for everyone. No song deals with the anger that this man has. He is looking for peace and comfort of his soul. He knows he is getting old without a wife and a job, he has to prospects. The first Yiddish song chosen is Oyfn Pripetshik (Go My child, to cheder). He is looking for comfort by goi ng back to study. Studying also means reawakening. The man is trying to get rid of the evil in his life in America, the time spent in prison, the four years spent by himself. He is burdened from his life from exile. "From reading the Gemara, this will shield you from all evil" He already knows this but it will be in his home country. What the children learn in cheder help them understand the tears they shed in life's experience and the knowledge will comfort them because they will have the all that is needed to get them through the most difficult times in life. As the editor said perhaps he should return home to study Gemara and the rabbi will tell him to come back to the New Word. The song, Gey Mayn kind in cheder, says that he studying is the best profession in the world, he will be nourished and his soul will become resourced. He will have the security and honor what he is missing in life. The most difficult task for this man is to forgive what he has gone through and rely on the knowledge of his childhood. When you are tired of exile, get comfort in studying. The second song explains two major thoughts of this letter. His desire to go back to his roots and his desire to study gemara. He came to America with so much hope to start a new life and make something of himself. He gave up all his roots and his origins. He gave up study and the Sabbath, he stopped being religious. His life fell to the worst state when he was accused of having burnt up his property. Four years after his prison term, Vos Geven Iz Un Nito, written in 1926 by the American Meyerowitz, talked about getting old. "I suffer from a disease†¦They call it old age". Our man is 30 years old. He does not have a family and considers it too late. It was most interesting in the last stanza where the lyrics "when I chance to pass by a school, I weep tears". He goes on to say that the young do not realize what a chance they have to study and when they do it is already too late. He feels this way on his own with no prospect for the future. In Frieling translated as Springtime, it is a man who has lost his love, and has no more purpose in his life. Kaczerginski wrote this song to memorialize his wife in 1943. Much of the song talks about walking thru the ghetto. Our man lived in a naive ghetto thinking his wife was good and kind. He sees his barricaded house, he sees how destroyed it has become. This was after the ghetto has been liquidated. It can also be a metaphor for how the poet felt after his wife was murdered. This song parallels the life of our man. He needs the spring light to come back into his life and to give him hope. If it is a metaphor for his life, he is looking for a solution to his sorrow; Springtime will give him a reason to see an answer to find a solution to what his wife did to him. Springtime brings the flowers out. He can possibly find a way of forgiving her with the change of seasons from the harsh winter to the optimism of the sunshine and the flower s. Though he will not forget, he can pardon. and plotted against him. When he writes the

Impacts of Taxation on Small and Big Businesses Essay

Impacts of Taxation on Small and Big Businesses - Essay Example ne either by an individual who is self-employed in his or her own small business or by a person who owns, manages and is the employee of his or her own business. If the treatment of tax of the income is derived from such activities differs greatly depending on the legal form in which the businesses are conducted, then the system of tax is more likely going to have a strong influence on the ways small and bug businesses are structured. Without any good reasons for favouring one legal form over the other, such distortions ought to be avoided. This will need both the same treatment of income from employment and self-employment within the personal system of tax and a similar treatment of income derived from small business and from small unincorporated business within the whole tax system (Welsh & White 2001, pp.18 – 27). As well as having these variety of legal forms, a second fundamental reason as to why small business do present vital challenges for tax design is that the income derived from the activities of small businesses do reflects a mix of rewards for labour supplied by those who work for the business and returns to the supplied capital by those who invest in the business (Button 2009, pp.389–408). The United Kingdom business community makes a huge contribution to the treasury’s coffer every fiscal year in terms of tax contribution. In the previous financial year, a total of one hundred and sixty three billion sterling pounds was paid as taxes by the business that are operating in the UK. Breaking the contribution of the business further down shows that, in addition to paying tax on the profits, businesses also contribute. This is based on their roles as the owners of the property, consumer of goods and services, employers and the impact on the environment (Adams et al. 2008, pp.101–115). It is also important to note that businesses contribute to the efficient running of the system of tax and the economy of the country, on top of their direct

Diversion Program Essay Example | Topics and Well Written Essays - 500 words

Diversion Program - Essay Example Diversion program in the criminal justice system is a program run by a police department, court, a district attorney's office, or outside agency designed to enable offenders of criminal law to avoid criminal charges and a criminal record. In October 2007, World Sport Chicago with the cooperation of USA Boxing, Chicago Park District and Police Athlete league of Illinois, launched a program "Gloves not Guns" to encourage school children to pick up boxing gloves and not guns to deal with aggression. The purpose of the "gloves not guns" program is to save youth from offenses and street crimes. So they chose boxing as a tool by which students can take out all their anger and aggression in the ring. They can put off their stress in a legal way rather than indulging themselves in any unwanted acts. "Most importantly, this program will provide our kids with positive alternatives and keep them off the streets and away from the dangers of gangs, guns and drugs. By motivating young people to spend their spare time in the gym, rather than on the streets, we'll be using sports to change people's lives for the better." If we look at this program from the perspective of social process theories, we observe that all the factors of social learning theory, social control theory and social reaction theory are associat

The Relationship And Unity Of The Malaysian People Education Essay

The Relationship And Unity Of The Malaysian People Education Essay The new economy policy was introduced by the government to strengthen the relationship and unity of the Malaysian people. By introducing new economy policy, the number of job opportunities had increased irrespective ethnicity, thus improve the relationship between people from different races. Besides that, DEB also played the roles to reduce and eliminate the identification of each race based on their works and living place. For example, Malay people usually live and work in the village while Indian people live and work on the estate. This will result in less interaction between both races and unities between them will not success easily. DEB had overcome this problem by providing equal opportunity to all Malaysian. The national Education policy was set up as recommended by Razaks Report 1956. According to the KLIK website (n.d), the aim of this report was to establish a national education system that would promote the cultural, social, economic and political development accepted by the nation as well as making the Malay language as the national language and the main language used in school. After Razaks Report, the government introduced a few education reports that contained better recommendations or the upgraded version of the first one. Government always changed the recommendations and policies due to the current education system that keep changing along with the globalization. Years ago, people did not care about education as they were not afforded for it. Nowadays, as the government had introduced pre-school for a child at the age of 5 and 6 years old in order to provide them with formal education. Preschool is the suitable place to explore the students talents and help them to improve it. At this time, the students are young and always ready to learn something new. This is the time for the teacher and parents to train the kids to be responsible, well-manner and able to contribute to the country. Along with the development of the country, people started to realize that people that is well-educated have the power to be respected and get higher income compared to someone without education. A national principle known as rukunegara is the basic principles that must be obeyed by all Malaysian. National principle is used to promote social balance and unity among different races that live in Malaysia. By following this principle, we can prevent from the tragedy 13 may from happen again. All the five principle focused on the relationship between man and god, king, country, law and constitution. The last part of the principle is good behavior which is reaching the goal of NPE to develop various potentials among student. By practicing it correctly, we may produce well potential students in various aspects, thus improve the quality of our country. Nowadays, people started to realize that education in school is not about performing well in the exam only but students have to involve in co-curriculum activities in order to identify their talent while train them to be more discipline. There are a few factors that lead to the NPE such as the factor of religious, social, politic, economic and individual. As stated in the first national principle which is belief in God, all the citizens supposed to have a religion to be obeyed. In Malaysia, the official religion is Islam. Even though Malays people are Muslims, which does not mean other people from different ethnic cannot join the religion. Besides that Muslims, other people are free to embrace any religion that they want. As Malaysia has multiracial citizens, social factor is one of the important factors that lead to NPE. In Malaysia, we can communicate and live happily with other ethnics as we respect each other as the citizen of the same country. All of them are living or work at the same places without arguing because the spirit of unity as stated in the second national principle which is loyalty to king and country. Each person has different skills in developing the national economy. For example, there are some people that mastering business while the others are skillful in buying and selling stock. This differentiation occurs due to different potential and education level that they got. When they are knowledgeable, they know how to compete in order to get profit as well as improve the economy level of the country. The last factor is individual factor. At school, students talent will be identified in order to develop their skills and potentials that are different between each person. Living in a community with a good manner will result in harmony and balance citizens. Besides that, their potentials and skills may help the country development in various aspects such as education, music and others. Education in Malaysia has started years ago. Formal education in Malaysia used to take place at the mosque and castle before changing into a place called school. Before independence, people that live in a rural area with low income rarely have the opportunity to go to school as they were not afforded for it. The developing of this country has improved the education in Malaysia thus provide a better opportunity for children to get education. Before 1982, the education system that had been used in Malaysia was Old Primary School Curriculum. As stated by fiq2705 (2012), the syllabus introduced a lot of subjects thus make it not suitable for a student in primary school. Besides that, this system was fully depended on textbooks with limited teaching aids and references. The student will get bored easily as the teacher was not creative to make the teaching and learning process more interesting. As the result of this, many people had criticized the education system at that time due to a lot of problems occur. To overcome this problem, the government had introduced New Primary School Curriculum. New Primary School Curriculum also known as Kurikulum Baru Sekolah Rendah (KBSR) had upgraded and improved in many ways better than the system before. As stated by are_niece02 (2010), the main focus of KBSR were to implement 3M among students which is reading, writing and counting. The students were divided into two phrases. Phrase 1 contained the Year 1 until 3 students while year 4 to 6 students were in phase 2. Each phrase stresses on different skill and knowledge suitable to the student age. According to are_niece02 (2010), the syllabus that had been practiced during KBSR was flexible for students as they balanced the emotional, spiritual, intellectual during the teaching and learning process. Due to this, on-going assessment had been introduced in order to identify the student performance and problems occur during the study process. Besides that, the teacher was requested to be more creative and not totally depended on text books. One of the problems occur during this program we re the lack of the capable teacher in certain subjects, thus they need to work more than other teacher. Role of a teacher is to teach. Teachers will felt confidence with the abilities that they have in certain subject. Headmasters role is to match the teachers abilities. Besides that, they play the biggest role in developing the potential of students and teachers in various aspects. For better understanding, i have made some research at a Sekolah Kebangsaan Putrajaya Presint 9 and meet with their headmaster, senior assistant and teacher.There are some activities that had been held at the school on to achieve NPE goal such as gotong-royong, Merdeka day celebration, Prophet Muhammad celebration, choral speaking competition and sports day. Gotong-royong is a compulsory activity that the students must join on every Monday before the assembly. During this activity, students are divided into a few groups. Each group will be accompanied by 3 teachers and have their own place to do this activity. Teachers are responsible to give instructions and guide the students. At the same times, they will make sure that the activity runs smoothly with any undesirable thing happen. The task for each group is they must clean up the place and make sure everything is well organized. Besides that, there is also a time when they will paint the school building. In order to make the activity more interesting, the school will give free drink on that day. The objective of this activity is to make them feel equality. No matter which family they are from, no matter which class they are, all students required to join this activity. It is one of the ways to train the students to be patient and tolerant while doing something. Without struggle, nothin g can be success. Besides that, students will be more discipline and follow the instruction given. Usually, when this activity finished, the students will take care of their area even the program was over. This activity has improved the students in many ways. They take good care of their surroundings to make sure that it will be clean thus people can live peacefully. By applying this in a community, it will improve the relationship between neighbourhood thus strengthen the unity. The objective of Merdeka Day Celebration is to make the student appreciate and proud with our country. Nowadays, kids and teenagers spend a lot of time with the machine more than having interaction with human beings. They usually play video games, surf internet and Facebooking during their free time. Spending less time with the older prevent them from learning about the things happen in the past. They just know the history based on what had been taught in the classroom. So, by holding this activity in the school, it can enhance the student knowledge about the life before independent. During this activity, teachers play their role to decorate the hall and preparing some suitable materials. Some of the students will be helping the teachers in the hall while the others will join poster competition. They are requested to draw something related to independence day and the winner will be announced on the day of the celebration. The poster activity goal is to make the students show their cr eativity and at the same time appreciating our country. Early in the morning, all students and teachers will gather in the hall to sing the national anthem, and the school song. After that, there will be a speech by a person that had the experience living during before independent. By exposing the story to the students, they are able to experience and imagine the life before. Thus, they realize how grateful they are nowadays. To solve the problem occur between ethnics, they will improve the social interaction between different races by respecting each other. This situation achieves the goal of NPE which is to produce balanced individuals. Another celebration held in the school is the Prophet Muhammad birthday celebration or also known as maulud. The objective of this celebration is to make the student remember the prophet Muhammad that had left us years ago. In this activity, teachers are responsible to prepare some materials such as Quran, inviting Ustaz and others. Besides that, the students will participate in some competition such as Tadarus Al-Quran, Tilawah Al-Quran, Story Telling and others. This activity was held in order to develop student potential in Islamic ways. During the celebration day, each class has to participate in banner decoration to develop their creativity. Usually, the banner will be written in jawi, thus this activity had improved their jawi writing. By the end of this activity, students are not only enjoying it but they learn more about the story of the prophet, how Islam develops and at the same time improve their skills in reciting al-Quran. So, by practicing it correctly, we can produce p eople that are not only excellent with academic but well-develop in religion. Having high intellectual but without the application of noble character could bring to the worst of the society and the nations (TIM 2011) The next activity held Coral Speaking Competition. The objective of this activity is to increase the English usage of the student and at the same time improve their vocabulary and pronunciation. Teachers are responsible to train the students one each class. Together with the students, the teacher will discuss and prepare the texts for their team. Each class will compete with each other and the winner will be getting a present during closing event. The theme of the choral speaking are different in each year depend on the current issue. So, at the same time, students learn and know about the thing happen in their surroundings. On the day of the competition, each team will present their choral speaking with their own creativity such as use fancy cloth, funny text and others. By the end of this activity, the student is not only enjoyed it but they also improve their English. English is an international language that is been spoken by people around the world. By holding this activity ever y year, students will be more competitive to be the best in English. Thus, they can communicate with others from different country and enhance their knowledge and at the same time strengthen international relationships between others. The last activity is sport day. As stated in Utusan online ( 2013) , students are starting to join sport and curriculum since 1985 and now the government had introduced Satu Murid Satu Sukan (1M1S). Joining this activity will improve the students psychomotor, cognitive and effective. The student may develop potential in the sport that they like. Teacher are responsible to prepare the place that the activity will be held. At the same times, students will join training to prepare themselves for the competition. Student are divided into 4 groups. Some one of the activities take place on that day are 100m sprint, broad jump and others. The Co curriculum had improved the student knowledge as well as train them to conduct and manage an activity. It has achieved the goal of NPE which is to produce creative students. Students also will look at school as interesting place that develop their skills and potential. The interaction in class is really important. In this area, the teacher plays the biggest role to communicate and help students in different ways. As an English teacher, there are some activities that are suitable to be conducted in class such as an individual teaching guide, quiz, ICT activities, spelling bee, and story telling. During teaching and learning process, here will be some students that are slow learners. This student cannot catch up what the teacher teaches. So, to help them understand the lesson. The teacher will spend special time for them before starting a new lesson. They can ask the teacher directly anything that they do not understand. By doing this, there are no students left out during the teaching and learning process. Using ICT programs in the classroom will attract the students interest and make them understand easily. ICT programs occupy all the learning style since there will be sound, picture, and activities that they have to do to enhance the understanding. Conducting quiz will be useful because the teacher can analyze which part or question that most of the students get wrong. Besides that, students will improve their understanding level. Spelling bee and storytelling is the activities that bring many advantages to the student. By joining this activity, they can improve their English. Besides that, it encourages student being competitive in order to be the best. Spelling bee improve their vocabulary and pronunciation. On the other hand, story telling improve the memorizing skills and their creativity to tell a story. Both activities help them in their studies. For example, there are some subject that need them to memorize the formula. So, by introducing this activity to the student, student are able to apply it in real life. In conclusion, the National Education Philosophy (NPE) plays the biggest role in developing education in Malaysia. It introduces various concepts in education, curriculum, co-curriculum and others. Besides that, NPE act as a guide for a teacher to improve their teaching style and activity in order to achieve the school objectives. As a whole, the changed in education as occur due some people opinions about education and the benefit of being educated. As stated in NPE, the objective of it is to develop physical, intellect, emotion, spiritual and social of the citizen to betterment. As a future educated, it is my responsibility to understand the contain of NPE and use it as a guide during my teaching lesson.

Human Cloning :: miscellaneous

Human Cloning Science fiction authors have for many years instilled in us the fantastic idea of great armies of cloned men and women, fighting mindless battles for the betterment of human kind. Perfect beings created under the microscope of fantasy to accelerate the evolutionary process, a brave new world, of disease free and identical people. But is there truly an application for human cloning in our 21st century society? Some scientists argue that one could use the cloning process to grow a twin sister for a dying child to allow for an organ donor, a perfect match. A noble idea, if one values a human life as highly as cattle headed for a slaughter house. And what of the dangers involved? A few years ago the world’s eyes turned to the scientific presses, hot with the new discovery that cloning was possible. Dolly the sheep was born, the first successful case of cloning the scientific world had seen. But what we didn’t see is that there were 276 failures before the successful case was achieved. Are we willing to gamble 276 human lives for the research of a non – applicable science? The thought of a perfect being is intriguing. The model child with the blue eyes you never had and the perfectly straight – without having spent three hours in a salon – blond hair that everyone thinks you have. It seems a popular notion that once one genetic modification has been achieved, others will follow. And even if the technology for a bouncing blue eyed catalogue selection is only a gleam in the scientific eye, the possibility of having a three year old Britney Spears is conceivable. The excitement of this sentiment unfortunately masks the reality of it, in that creating a homogeneous race poses a real threat to freedom, the very essence of humanity. At this stage in the development of the cloning process, each cloned Being is viewed as a subject. Dolly was a media spectacle, a lost lamb under the millions of gawking eyes. If a human is cloned, it is highly unlikely that he or she will not be swept up into a similar fate. And under the eyes of the media, not to mention the person who funded the â€Å"subject†, that child will be forced to grow up under a rock of obligatory expectations. Every action and emotion could indeed be shaped and cultivated to suit the perceptions of an idealised person.

Immune System and Normal Red

Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? 1. Case 1: Sickle-Cell Anemia 2. Case 2: Mononucleosis 3. Case 3: Pernicious Anemia Journal Questions for lab 1. In what ways do normal red and white blood cells differ? Normal blood cells carry oxygen throughout the body. White blood cells fight against viruses, infections, and other foreign objects. 2. Which type of white blood cell would you expect to be most common in a normal blood smear?Neutrophils 3. A differential count of white blood cells from a patient gave the absolute number of lymphocytes as 8000 per mm3 and the total number of white blood cells as 12,000 per mm3. Calculate the percentage of lymphocytes in this sample of white blood cells. Is this a normal or abnormal percentage? Explain your answer. You divide 8000 by 12000 and then times by 100. This will give you 2/3% (66. 67%) . this is abnormal regular is 20-40% 4.Describe the difference between a communicable disease and an inherited disease. Use examples you have studied in this exploration to support your description. A communicable disease can be transferred for one person to one another. A inherited disease is passed by genetics. 5. Why white blood cells in a stained blood smear are usually counted at low power under a microscope? Explain your answer. Because they are bigger than other blood cells. . Why is the presence of a larger than normal number of neutrophils indicative of an infection? Explain your answer. Cause neutrophils fight infections so if there is a higher than normal that means that they are fighting off an infection. 7. Why would you not expect to see tissue macrophages in a blood smear? Explain your answer. Cause macrophages move within the tissue unless tissue is in the blood smear.

Cause of the Civil War essays

Cause of the Civil War essays The Civil War is commonly thought to have been caused by the idea or institution of slavery in the United States. Although slavery did play a part in leading to the Civil War, the true cause of the United States of America being divided into two separate entities was succession. More specifically the succession of the state of South Carolina from the Union. The succession of South Carolina, the catalyst which ignited the conflict of the Civil War, was brought of by two key factors, the southern nationalistic views and the strong leadership among the Cotton Kingdom1 which South Carolina possessed. Ten years prior to the Civil War the south, especially South Carolina, had a strong feeling of southern nationalism. This was of no surprise considering all of the influences and events of the time period, 1850. The historical meeting of the Nashville Convention, November 14, 1850, was of the largest importance dealing with this issue. Cheves launched an attack on Henry Clays Compromise of 1850, saying to the southerners, Unite, and you shall form one of the most splendid empires on which the sun ever shone, of the most homogeneous population...2. Cheves went even further, making a defensive attack on how the south should unite and further declined that the institution of slavery was not wrong or immoral, but in fact was apart of their culture and their society. Chevess speech at the convention was not the only topic that brought together and connected the people of the Palmetto State3 and the rest of the south, but the tariff and nullification episode between 1827 and 1833 also gave the southerners a feeling of nationalism to their part of the country. The tariffs gave the south such a disadvantage to the north that they had no other choice but to band together. Cotton, the souths most lucrative and essential industry, price, ...had fallen from thirty cents a p...

Rise of Hitler source study Essays - Free Essays, Term Papers

Rise of Hitler source study Essays - Free Essays, Term Papers Rise of Hitler source study Source 1 A 1924 right-wing German political cartoon showing Philipp Scheidemann , the German Social Democratic politician who proclaimed the Weimar Republic and was its second Chancellor, and Matthias Erzberger , an anti-war politician from the Centre Party, who signed the armistice with the Allies, as stabbing the German Army in the back Source 2 "The older section of the middle class, comprising artisans, small retailers and peasant farmers, formed the core of the support for Hitler, and were showing support for him before the Depression; theirs was a disillusionment with the structure and policies of the Republic itself. To these was subsequently added the weight of much of the new middle class- the non-manual employees, civil servants and teachers- who aligned themselves with Nazism as a direct result of the Depression. Stephen Lee, historian Using s ource one, explain how some German s view ed the Weimar Republic . 5 marks Using the sources and your own knowledge, examine reasons why some Germans believed the Weimar government had stabbed them in the back'. 10 marks Using the sources and your own knowledge, analyse reasons why Germans supported Hitler . 10 marks

Seth Godin and his impact on modern marketing practices Research Paper

Seth Godin and his impact on modern marketing practices - Research Paper Example In fact, Godin has introduced the concept that marketing can play two key roles – in addition to its existing functions worldwide. It is explained that marketing can be used in order to sell products/ services to people of quite low income – reference is made to the markets of the emerging countries where the average level of income is quite low, compared to the developed countries (Fawkes 2010). It is further emphasized that the exclusive use of marketing in developed countries cannot be considered as acceptable leading to the promotion of social exclusion and discrimination. Instead, marketing should be also used in emerging economies for promoting products that are aligned with local needs. Another characteristic of the views of Godin on marketing is the following one: Godin believes that marketing is not just a business activity; rather it is a framework that can ‘change the world’ (Fawkes 2010). This target is achieved, in accordance with Godin, in the following way: by improving the quality of products sold, entrepreneurs increase their chances for success; if the products offered in the market are of good quality, consumers are more likely to buy them developing a trend based on the increase of quality/demand, as a characteristic of modern market. The existing practice of low price/ low quality products would be then eliminated changing market values and trends. At the next level, Godin supports that the individual who wishes to develop his career in marketing should start working on the specific field independently; then, he can try to enter a firm that operates in the particular sector; as Godin states ‘if you get great at it, you’ll discover jobs find you’ (Sakas 2010). Also, Godin considers marketing as a responsibility, and not as a right... To the question of Rachelle Money how his involvement in marketing is explained, Godin refers to one of his key characteristics: he ‘enjoys watching his ideas spread’ (Money 2008). It is perhaps in this context that Godin has tried, successfully, to develop terms that represent key marketing functions and characteristics. Through the issues highlighted above it is made clear that the success of Godin in the area of marketing can be considered as resulted because of the interaction of many different factors: personal views, experiences and knowledge. In accordance with the issues discussed above, the main difference between Godin and the supporters of the traditional marketing theorists are their views on the role of consumer in the success of a marketing campaign. As Godin highlighted, the success of each marketing strategy is depended on its ability to address the needs of consumer – a fact that should be made clear to the customer, not just hypothesized. The success of Godin in the particular field is related with a series of facts: a) the level of his knowledge on marketing – Godin holds a Stanford MBA, b) his long term experience in marketing – referring to his work as ‘a marketing director of Whole Food Market and vice-president of the direct marketing department in Yahoo’ (Business Week 2008), c) his innovative ideas on marketing, as explained above, which have introduced the following trend: marketing campaigns are aligned with the preferences of customers and not with the need of busine sses for improving their performance. Moreover, Godin has helped towards the improvement of existing marketing concepts; indeed, the views of Godin, as reflected in his Permission Marketing concept, has been the basis for the update of common marketing policies, like the e-mail marketing and the content marketing, in the context described above.

Organ Transplant Research Paper Example | Topics and Well Written Essays - 1000 words

Organ Transplant - Research Paper Example In the U.S alone over 28,000 patients under transplant surgeries and over half of these surgeries are kidneys transplants while a quarter are liver transplants. Process of Organ Transplantation Organ transplantation calls for therapeutic usage of organs to replace a non-functional organ. However, the medical process is quite complex since organ transplantation comes with its own bag of complications and challenges. Keeping aside the legal issues and paperwork involved, the first step i.e. procurement of a healthy donor is a mammoth task since it care should be taken that the blood types of the recipient as well as the donor matches since â€Å"fate of a graft depends not only on excellent surgery with avoidance of damage to the organ in the process, but, as indicated above, the degree of HLA matching of donor and recipient is crucial in any approach to tolerance† (Calne, 2005, p1979). One a suitable candidate is found the healthy organ is harvested carefully and the damaged or gan in the recipient’s body is surgically replaced with the new healthy organ. Post-surgery, the newly replaced organ is kept under observation to detect rejection by the recipient’s body. Immunosuppressant drugs are administered to help the body accept the new organ and gradually integrate it in the patient’s body as its own. ... Such phenomena lead to organ rejection or blood transfusion reaction. Heavy immunosuppressant drugs are therefore administered after a transplant surgery. Therefore, it is necessary to match the donor and receiver’s blood so that the organ acceptation by the receiver’s body is smoother. The rejection mechanism of allograft kidney transplantation was studies and it was seen that â€Å"the most common form of acute allograft rejection is initiated when donor alloantigens are presented to the T lymphocytes of the recipient by antigen-presenting cell (APC’s) â€Å"(Schwartz, 2010, p1453). A new study shows that Jagged2 plays an important role in accelerating heart transplants or cardiac tissue grafts since its signaling affects the acceptation rate of graft by regulating interleukin-6 (Riella et al, 2013, 1449). Ethics and Importance of Organ Donation Becoming a donor is a tough choice for many. There are 2 types of donor-living donor and deceased donor. Organ dona tion is an important choice that can save a lot of lives however, organ donation, in many parts of the world, has rapidly emerged into a trade. For example, poor people sell one of their kidneys to the rich and since The risk involved in nephrectomy is not in itself high, and most people regard it as acceptable for living related donors (Radcliffe, 1998, p1951). However some people find the procedure too invasive and thereby refuse the whole idea of organ transplantation (NHMRC,1997,P12). Brain death and organ donation Organs are procured from cadavers however brain dead individuals provide fresher organs for harvest. This, stirs up an ethical issue since complete loss of brain function needs to